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MV suppressed inflammatory responses. ( A ) The levels of IL-6, IL-18, IL-1β, TNF-α <t>and</t> <t>MCP-1</t> in the supernatant were detected using ELISA after treatment with MV (25, 50, 100 mg/kg/d) or Met (101.4 mg/kg/d) in the serum of db/db mice (n = 3). ( B ) The levels of TNF-α, IL-1β, MCP-1 and IL-18 in the supernatant were detected using ELISA after treatment with HG in the presence or absence of MV or Met for 48 h in HRMC. ** p < 0.01 vs. respective control group; # p < 0.05 vs. respective DKD group (DKD mice for panel A, HG-treated HRMC for panel B); ## p < 0.01 vs. respective DKD group.
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MV suppressed inflammatory responses. ( A ) The levels of IL-6, IL-18, IL-1β, TNF-α and MCP-1 in the supernatant were detected using ELISA after treatment with MV (25, 50, 100 mg/kg/d) or Met (101.4 mg/kg/d) in the serum of db/db mice (n = 3). ( B ) The levels of TNF-α, IL-1β, MCP-1 and IL-18 in the supernatant were detected using ELISA after treatment with HG in the presence or absence of MV or Met for 48 h in HRMC. ** p < 0.01 vs. respective control group; # p < 0.05 vs. respective DKD group (DKD mice for panel A, HG-treated HRMC for panel B); ## p < 0.01 vs. respective DKD group.

Journal: International Journal of Molecular Sciences

Article Title: Mogroside V Alleviates Renal Injury in Diabetic Mice via Regulation of the TLR4 / NF-κB Pathway and Modulation of ECM Remodeling

doi: 10.3390/ijms27146271

Figure Lengend Snippet: MV suppressed inflammatory responses. ( A ) The levels of IL-6, IL-18, IL-1β, TNF-α and MCP-1 in the supernatant were detected using ELISA after treatment with MV (25, 50, 100 mg/kg/d) or Met (101.4 mg/kg/d) in the serum of db/db mice (n = 3). ( B ) The levels of TNF-α, IL-1β, MCP-1 and IL-18 in the supernatant were detected using ELISA after treatment with HG in the presence or absence of MV or Met for 48 h in HRMC. ** p < 0.01 vs. respective control group; # p < 0.05 vs. respective DKD group (DKD mice for panel A, HG-treated HRMC for panel B); ## p < 0.01 vs. respective DKD group.

Article Snippet: ELISA kits for TNF-α (Cat: EK182), IL-1β (Cat: EK101B), IL-6 (Cat: EK106), MCP-1 (Cat: EK187), and IL-18 (Cat: EK118) were purchased from Hangzhou Lianke Biotech Co., Ltd. (Hangzhou, China).

Techniques: Enzyme-linked Immunosorbent Assay, Control

Effect of MV on inflammatory factors. ( A ) Western blot analysis was used to detect the expression of inflammatory proteins TNF-α, IL-1β, and IL-18 in db/db mice. ( B ) Western blot analysis was used to detect the expression of inflammatory proteins TNF-α, IL-1β, MCP-1 and IL-6 in HRMC. ** p < 0.01 vs. respective control group; # p < 0.05 vs. respective DKD group (DKD mice for panel A, HG-treated HRMC for panel B); ## p < 0.01 vs. respective DKD group.

Journal: International Journal of Molecular Sciences

Article Title: Mogroside V Alleviates Renal Injury in Diabetic Mice via Regulation of the TLR4 / NF-κB Pathway and Modulation of ECM Remodeling

doi: 10.3390/ijms27146271

Figure Lengend Snippet: Effect of MV on inflammatory factors. ( A ) Western blot analysis was used to detect the expression of inflammatory proteins TNF-α, IL-1β, and IL-18 in db/db mice. ( B ) Western blot analysis was used to detect the expression of inflammatory proteins TNF-α, IL-1β, MCP-1 and IL-6 in HRMC. ** p < 0.01 vs. respective control group; # p < 0.05 vs. respective DKD group (DKD mice for panel A, HG-treated HRMC for panel B); ## p < 0.01 vs. respective DKD group.

Article Snippet: ELISA kits for TNF-α (Cat: EK182), IL-1β (Cat: EK101B), IL-6 (Cat: EK106), MCP-1 (Cat: EK187), and IL-18 (Cat: EK118) were purchased from Hangzhou Lianke Biotech Co., Ltd. (Hangzhou, China).

Techniques: Western Blot, Expressing, Control